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Double phosphorylation of phosducin-like protein on the 13–32 peptide. Collison-induced dissociation spectrum, chosen by hand for LC-MS/MS, of the parent ion at m/z 1321.5 is shown, representing amino acids 18–32 plus two phosphates. Fragment ions support identification of this peptide, but do not allow delineation of the phosphorylation sites within the peptide. The doubly charged fragment ion at m/z 1240 is a result of two lost phosphates.
