TABLE 1.

Optimization of Acetonitrile Precipitation

	STANDARD METHOD	MODIFIED METHOD
Mixing after addition of acetonitrile	Gentle Mixing	Vortexing
	Cloudy supernatant	Clearer supernatant
	Higher protein concentration	Lower protein concentration
	Inconsistent protein concentration	Consistent protein concentration
Centifugation	4 min at 12,000 rpm	10 min at 12,000 rpm
	Cloudy supernatant	Clearer supernatant
Concentrated volume of supernatant	50 μL	200 μL
	Precipitate forms	No precipitate forms