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Phosphopeptide isolation and enzymatic verification by usage of Gyrolab MALDI IMAC1 before MALDI analysis. A: 30 fmol β-casein digest, 270 fmol BSA digest. B: dephosphorylation of sample depicted in A. C: 30 fmol β-casein digest, 2970 fmol BSA digest. D: dephosphorylation of sample depicted in C. Phosphopeptides are annotated with p, dephosphorylated phosphopeptides with dp.
