FIGURE 3.

Western blot analysis of equal protein amounts collected following continuous-flow ultracentrifugation of rat liver homogenates from (a) 8- to 10-wk-old male and (b) greater than 1-y-old male Sprague-Dawley rats. One fraction showing the highest intensity gel band in the juvenile rat liver homogenate (Fraction 19) was selected for further analysis. Fraction 19 in the aged rat liver homogenate, which also shows the highest intensity band, was selected for comparison. “d” refers to sucrose density of the fraction.