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. 2005 Dec;16(4):347–355.

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Copyright © Copyright 2005 by The Association of Biomolecular Resource Facilities

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Western blot analysis of equal protein amounts collected following continuous-flow ultracentrifugation of rat liver homogenates from (a) 8- to 10-wk-old male and (b) greater than 1-y-old male Sprague-Dawley rats. The fraction showing the highest intensity gel band in the juvenile rat liver homogenate (Fraction 8) was selected for further analysis. Fraction 10 in the aged rat liver homogenate, which also showed the highest intensity gel band, was selected for comparison. “d” refers to sucrose density of the fractions.