TABLE 1.

DNA Sequencing Reaction Conditions Used to Generate the Top Three Quality Scores for Templates A, B, and C by Instrument

Template	Instrumenta	Rank	Quality Score (q20)	WTR Length (cm)b	Primer (pmol)	Template (μg)	Reaction Volume (μl)	DNA Sequencing Chemistryc
A	377	1	837	48	3.2	300	20	BDTv3.1
		2	713	48	15.0	1140	40	BDTv3.1
		3	690	48	3.2	300	20	BDT v3.1
	3100	1	815	80	12.5	380	10	BDTv3.1
		2	765	80	5.0	630	20	BDTv3.1
		3	765	80	3.2	494	20	BDTv1.1
	3700	1	767	50	10.0	200	10	BDTv3.1
		2	715	50	10.0	200	10	BDTv3.1
		3	708	50	10.0	200	10	BDTv3.1
B	377	1	951	48	4.8	400	20	dGTpv3.0
		2	856	48	3.2	300	20	BDTv3.1
		3	793	48	5.0	800	20	dGTpv3.0
	3100	1	879	80	5.0	730	20	BDTv3.0:dGTpv3.0 (4:1)
		2	871	80	3.2	494	20	BDTv3.0:dGTpv2.0 (2:1)
		3	854	80	5.0	730	20	dGTpv3.0
	3700	1	784	50	4.0	200	15	BDTv3.1
		2	767	50	3.5	365	10	BDTv3.1
		3	758	50	3.5	365	10	BDTv3.0
C	377	1	908	48	4.8	400	20	dGTpv3.0
		2	820	48	3.2	300	20	BDTv3.1
		3	729	48	15.0	2550	40	dGTpv3.0
	3100	1	881	80	5.0	850	20	dGTpv3.0
		2	844	80	5.0	850	20	BDTv3.0:dGTpv3.0 (4:1)
		3	835	80	3.2	494	20	BDTv3.0:dGTpv2.0 (2:1)
	3700	1	805	50	10.0	400	10	BDv3.1
		2	788	50	10.0	400	10	dGTp v3.0
		3	777	50	4.0	200	15	BDT 3.1

^aInstrument: Applied Biosystems Models 377, 3100, and 3700 sequencers.

^bWTR Length: well-to-read length (cm).

^cChemistry: Applied Biosystems ABI Prism big dye terminator (BDT) and dGTp big dye terminator ready reaction mixes. Parentheses indicate the ratio of BDT to dGTp.