TABLE 2.

Summary of Domains Identified from Protein Fragments

Protein	Domain	In vitroa	In vivoa
Chains generated by known processing pathways for enzyme activation
Cathepsin D	8–9: heavy chain
Chymotrypsinogen B	17–18: neo-chymotrypsinogen A-B chain 19–20: chymotrypsin B chain		20–22
Protein fragments corresponding to structural domains
GRP78	22–23: N-terminal ATPase domain 24: C-terminal oligomerization domain	24, 25, 39	23
Pancreatic lipase related protein 2	31: N-terminal catalytic domain	31, 32	30, 33
Prolyl 4-hydroxylase β	35: a (thioredoxin domain)-b-b′ 36: a-b	26–28	29
Heat shock cognate protein 71 kDa	20: N-terminal ATPase domain with NLS, lacks NES and NLRS sequences, possible inhibitory variant like 54 kDa variant which is not able to move into nucleus but remains in cytoplasm even under stress.	40	7, 8
Cytokeratin 8	43: linker and coil 2 CK18 binding domain. Similar to cleavage produced by limited proteolysis studies where 20 kDa coil 2 generated with trypsin and chymotrypsin	34	9, 34, 41–42
Actin, β and γ	59: C-terminal subdomains 3 and 4	43
Protein fragments not associated with domains
α-amylase 2A		36–38
14-3-3-ζ

^aReferences are listed for previous observation of protein fragment domains for the proteins listed, either in vitro or in vivo.