Skip to main content
. 2006 Sep;17(4):283–292.

FIGURE 1.

FIGURE 1

FIGURE 1

Cloning strategies used to construct the Tet-On system. A: Schematic representation of the cloning steps used to make the regulatory plasmids pCMV-rtTA and pNSE-rtTA. in PCMV-rtTA, expression of rtTA is driven by the CMV promoter, whereas in pNSE-rtTA, the CMV promoter of the pIRES2-EGFP plasmid was replaced with the NSE promoter and the rtTA fragment subcloned downstream of the NSE promoter after removing the EGFP fragment. B: Schematic illustration of the subcloning steps involved in the construction of the response plasmid. The CMV promoter was replaced with TREtight in the pIRES2-EGFP, and the gene RAGE subcloned downstream of the TREtight.