TABLE 1.

Primer Specifications and PCR Conditions

Target		Primer sequences (5′ → 3′)	PCR conditions	Product size
NSE	Forward(AseI site at 5′)	attaatgagctcctcctctgctcgc	94°C × 4 min	1.8 Kb
	Reverse(NheI site at 5′)	gctagcctcgaggactgcagactcag	35 Cycles 94°C × 1 min 54°C × 1 min 72°C × 2 min Extension: 72°C ×10 min
rtTA	Forward (NheI site at 5′)	gctagcatgtctagattagataaaagtaaagtgatt	Same as above	1.0 Kb
	Reverse (EcoRI site at 5′)	gaattcctacccaccgtactcgtca
TREtight	Forward(AseI site at 5′)	attaatctcgagtttaccactccctatcag	94°C × 4 min	0.362 Kb
	Reverse(NheI site at 5′)	gctagcaggatccccaggtaccgt	35 Cycles 94°C × 30 sec 54°C × 30 sec 68°C × 1 min Extension: 68°C × 10 min