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. 2006 Dec;17(5):308–326.

TABLE 4.

Relative Retention Times of Standard Amino Acids Normalized to Ala and Scaled to the Average Run Time

494-Ht Instruments (n = 19) 494-cLC Instruments (n = 8)
494-HT Av RTnAs 494-HT Std Dev of RTnAs 494-HT Av Full RT 494-cLC Av RTnAs 494-cLC Std Dev of RTnAs 494-cLC Av Full RT
D −0.30 0.03 4.20 −0.30 0.01 6.18 D
N −0.27 0.02 4.62 −0.27 0.01 6.60 N
S −0.22 0.01 5.25 −0.23 0.01 7.35 S
Q −0.20 0.01 5.52 −0.20 0.01 7.69 Q
T −0.18 0.01 5.75 −0.19 0.01 7.92 T
G −0.17 0.01 6.00 −0.17 0.00 8.24 G
E −0.13 0.01 6.48 −0.13 0.00 8.79 E
H −0.04 0.02 7.69 −0.05 0.01 10.12 H
A 0.00 0.00 8.22 0.00 0.00 10.89 A
R 0.12 0.02 9.78 0.11 0.01 12.54 R
Y 0.16 0.01 10.36 0.16 0.01 13.38 Y
P 0.33 0.00 12.62 0.33 0.00 16.06 P
M 0.38 0.01 13.34 0.38 0.01 16.87 M
V 0.41 0.00 13.66 0.41 0.00 17.24 V
W 0.55 0.01 15.56 0.57 0.01 19.87 W
F 0.63 0.02 16.65 0.63 0.01 20.70 F
I 0.66 0.02 17.09 0.66 0.01 21.20 I
K 0.65 0.03 16.90 0.68 0.01 21.53 K
L 0.70 0.03 17.62 0.70 0.01 21.83 L