TABLE 5.
Relative Retention Times of Modified Amino Acids Normalized to Ala and Scaled to the Average Run Time for Each Group of Instruments
| ABI 494- HT | ABI 494-cLC | ABI 477 (n = 1) | Porton (n = 1) | ||||||||||||||||
| Cycle | Amino Acid | Average SRTnAs | Std Dev of SRT-nAs | Av Full RT | Rel Pk Area | Rel.Area Std Dev | n | Average SRTnAs | Std Dev of SRT-nAs | Av Full RT | Rel Pk Area | Rel. Area Std Dev | n | SRTnA | 477 Full RT | Rel Pk Area | SRTnA | Full RT | Rel Pk Area |
| 1 | Tyr | 0.16 | 0.01 | 10.37 | 102.3% | 9.8% | 23 | 0.18 | 0.01 | 13.69 | 107.2% | 13.9% | 10 | 0.16 | 16.60 | 85.8% | 0.14 | 13.53 | 77.1% |
| 2 | Dimelys | 0.11 | 0.04 | 9.75 | 88.0% | 27.5% | 23 | 0.13 | 0.04 | 12.93 | 75.8% | 20.3% | 10 | 0.12 | 15.60 | 58.0% | 0.22 | 14.70 | 116.4% |
| 3 | Ala | 0.00 | 0.00 | 8.24 | 103.8% | 9.6% | 23 | 0.00 | 0.00 | 10.86 | 104.1% | 10.3% | 10 | 0.02 | 13.60 | 103.8% | 0.00 | 11.18 | 108.0% |
| 4 | 3MeHis | 0.01 | 0.07 | 8.35 | 85.9% | 36.7% | 21 | 0.03 | 0.05 | 11.33 | 67.7% | 29.5% | 10 | 0.02 | 13.50 | 71.6% | 0.10 | 12.77 | 129.6% |
| 5 | Lys | 0.61 | 0.03 | 16.46 | 140.3% | 48.8% | 22 | 0.77 | 0.01 | 22.86 | 107.0% | 51.4% | 10 | 0.68 | 27.80 | 7.1% | 0.67 | 21.93 | 143.8% |
| 6 | His | −0.04 | 0.02 | 7.69 | 60.3% | 16.9% | 23 | −0.05 | 0.01 | 10.13 | 51.8% | 21.3% | 10 | −0.03 | 12.40 | 35.5% | −0.05 | 10.50 | 178.1% |
| 7 | Homocit | −0.09 | 0.01 | 7.01 | 88.7% | 17.9% | 22 | −0.11 | 0.00 | 9.21 | 75.3% | 29.3% | 10 | — | — | — | −0.12 | 9.27 | 158.9% |
| 8 | Ala | 0.00 | 0.01 | 8.24 | 90.4% | 20.6% | 23 | 0.00 | 0.00 | 10.87 | 78.3% | 29.2% | 10 | 0.02 | 13.50 | 108.1% | 0.00 | 11.22 | 135.8% |
| 9 | Cystine 1 | 0.17 | 0.12 | 10.49 | 30.1% | 37.3% | 20 | 0.18 | 0.15 | 13.66 | 11.6% | 6.8% | 10 | — | — | — | 0.06 | 12.25 | 16.9% |
| 9 | Cystine 2 | 0.09 | 0.15 | 9.48 | 4.8% | 2.0% | 8 | 0.20 | 0.05 | 13.95 | 7.0% | 5.5% | 7 | — | — | — | 0.17 | 13.88 | 22.1% |
| 9 | Cystine 3 | 0.05 | 0.14 | 8.92 | 3.2% | 1.5% | 7 | 0.09 | 0.22 | 12.24 | 2.4% | 0.8% | 5 | — | — | — | — | — | — |
| 10 | Tyr | 0.15 | 0.01 | 10.27 | 104.3% | 13.5% | 23 | 0.18 | 0.01 | 13.69 | 94.9% | 20.2% | 10 | 0.16 | 16.50 | 129.9% | 0.15 | 13.60 | 115.1% |
| 11 | Trimelys | 0.11 | 0.06 | 9.63 | 65.9% | 31.2% | 19 | 0.21 | 0.05 | 14.16 | 56.9% | 40.1% | 10 | 0.19 | 17.10 | 41.8% | 0.31 | 16.20 | 115.2% |
| 12 | Gly | −0.15 | 0.01 | 6.15 | 75.6% | 16.6% | 23 | −0.19 | 0.01 | 7.89 | 70.0% | 20.6% | 10 | −0.14 | 10.10 | 130.1% | −0.17 | 8.58 | 99.7% |
| 13 | NmeAla | 0.21 | 0.02 | 11.03 | 84.3% | 29.5% | 22 | 0.26 | 0.01 | 14.99 | 71.3% | 20.7% | 10 | 18.00 | 51.2% | 0.25 | 15.30 | 109.9% | |
| 14 | Tyr | 0.16 | 0.02 | 10.30 | 110.3% | 15.5% | 23 | 0.18 | 0.01 | 13.68 | 118.1% | 18.0% | 10 | 0.16 | 16.50 | 84.9% | 0.15 | 13.63 | 87.7% |
| 15 | Ala | 0.00 | 0.01 | 8.24 | 91.9% | 20.8% | 23 | 0.00 | 0.00 | 10.87 | 98.3% | 13.8% | 10 | 0.01 | 13.30 | 93.9% | 0.00 | 11.25 | 87.5% |
| 16 | IsoAsp | — | — | — | — | — | — | — | — | — | — | — | — | ||||||