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. 2006 Dec;17(5):308–326.

TABLE 7.

HPLC Information

Column Manufacturer, Type, Size All 477A and all 49X-HT used ABI/Brownlee Spheri-5
PTH column 2.1 x 220 (or 150) mm
6 cLC used ABI/Brownlee Spheri-5 PTH 0.8 x 250 mm
1 cLC used ABI/Brownlee Spheri-5 PTH 0.8 x 150 mm
Column Age Ranged from 20 to 2000 cycles
Mean 611 cycles
Avg. Column life ABI 0.8 x 250 mm: 300–1,400 (mean 855)
ABI 2.1 x 220 mm: 400–3,000 (mean 1405)
Solvent A All used ABI Solvent A
Additives to Solvent A besides Premix Acetic acid/na Acetate/ 0.2% 1-hexane sulfonic acid

  • Na2HPO4/TFANa phosphate/acetone

  • Acetone only

  • TFA only

  • TFA/acetone

  • Trp/TFA/KH2PO4

  • TFA/KPO4/acetone

  • KH2PO4/acetone? Acetone/0.1m phosphate,pH5/1m NaOH
Solvent B All use ABI Solvent B2 (isopropanol/acetonitrile) or equivalent (some users are not clear whether they use ABI B2 solvent).