Table 1.
Antibodies directed against HOS3 are able to specifically immunoprecipitate deacetylase activity from nuclear extracts
| Yeast strain | Anti-HOS3 | Anti-HDA1 | Anti-RAP1 | Beads alone |
|---|---|---|---|---|
| Wild type | 1,140 | 5,888 | 191 | 108 |
| hos3Δ | 131 | 6,052 | 147 | 171 |
Activity is given as total cpm release from 50,000 cpm total [3H]acetyl Hela histones. Extracts were prepared, immunoprecipitated, and assayed as described previously (1).