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. Author manuscript; available in PMC: 2009 Mar 13.
Published in final edited form as: J Pharm Biomed Anal. 2007 Dec 17;46(4):692–698. doi: 10.1016/j.jpba.2007.12.014

Fig. 3.

Fig. 3

General workflow for the chemical subtraction of a target compound (X) from a total plant extract (T) or any other complex (natural) material: A suitable two-phase solvent system (SS) is selected from established or newly designed solvent system families. Utilizing an assay capable of measuring partition coefficients (K), the polarity of a selected SS family is then adjusted to match a suggested target values of K(X) ∼ 3. Subsequent HSCCC fractionation yields the target compound (X) at elution volumes that can be predicted using established CCC theory for elution [19, 37, 38] and extrusion [25, 39]. The surrounding fractions can be recombined to the chemically subtracted starting material, T-X (see discussion for further explanation and references).