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NIR imaging of in vivo scFv biodistribution at 4 h post-injection. (a) Specific targeting was seen with scFv 10A to the tumor treated with 6 Gy (left leg) as compared to untreated tumor (right leg) and the rest of the body as early as 4 h post-injection. (b) No tumor-specific binding seen with mice treated with scFv 4A.
