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. Author manuscript; available in PMC: 2009 Feb 1.
Published in final edited form as: Biochim Biophys Acta. 2008 Jan 7;1782(2):99–108. doi: 10.1016/j.bbadis.2007.12.003

Fig. 5. Expression of rab5 and rab11 mutants inhibit adiponectin secretion in 3T3L1 cells.

Fig. 5

Fully differentiated 3T3L1 cells were electroporated with a construct expressing adiponectin-myc (white bars) or leptin-HA (black bars) and either an empty vector, a wild type or constitutive active/inactive mutants of rab 11 (Panel A) or rab5 (Panel B). Following expression of the proteins, media and whole cell lysates were harvested and an aliquot of each were quantified by ELISA as described in the methods section. The amount of secretion is shown as the ratio to the secretion obtained in control adipocytes expressing each adipokine and the empty vector ± S.E. The graph represents data from three independent experiments and 18 independent observations. Differences were considered significantly different when p < 0.05 and is indicated with an asterisk. Expression of adiponectin-myc, leptin-HA and rab proteins were confirmed by western blot analysis (data not shown). For this ELISA we utilized an adiponectin capture and detection antibodies, respectively, as described before [35].