Table 1.

Transcript levels of viral genes on ISAV segment 8 in extended haemagglutination assays¹

Sampling points in days	NBISA01 haemagglutination		RPC/NB-04-085-1 haemagglutination
	One-step RT-PCR with F5/R5 primer pair	RT with oligodT primer & PCR with F5/R5 primer pair	One-step RT-PCR with F5/R5 primer pair
0	26.57 ± 0.14	22.22 ± 0.05	20.39 ± 0.21
1	24.43 ± 0.15	20.63 ± 0.05	21.23 ± 0.15
2	22.25 ± 0.21*	19.40 ± 0.02	20.46 ± 0.26
3	21.51 ± 0.33*	18.31 ± 0.02*	20.76 ± 0.36
4	21.04 ± 0.40*	18.67 ± 0.27*	20.27 ± 0.22
5	20.48 ± 0.29*	19.29 ± 0.12*	20.55 ± 0.29

¹Ct values are average ± SD of triplicate observation.

*denotes more than 3.3 Ct difference in the starting sample concentrations from that of the 0 hour.