Table 2.

The oligonucleotide primers, amplicon length and amplification efficiency of the real time RT-PCR primers amplifying IFN, Mx, 18S, ISG15 and STAT1 genes

Primer name	Primer sequence	Amplicon length (bp)	Amplification efficiency
As IFN Fwd1	TGCAGTATGCAGAGCGTGTG	100	1.83
As IFN Rev1	TCTCCTCCCATCTGGTCCAG
As Mx Fwd	TGCAACCACAGAGGCTTTGAA	77	1.88
As Mx Rev	GGCTTGGTCAGGATGCCTAAT
As 18S Fwd	TGTGCCGCTAGAGGTGAAATT	60	1.86
As 18S Rev	GCAAATGCTTTCGCTTTCG
As ISG15 Fwd	CTGAAAAACGAAAAGGGCCA	100	1.83
As ISG15 Rev	GCAGGGACTCCCTCCTTGTT
As STAT1 Fwd	TGTCTGTTGGCTCAGTTGCG	100	1.82
As STAT1 Rev	GAAATTGATGCTGTGGCGTCT
As PKZ Fwd	AGATAGCGAAGGCTGTTGGA	101	1.913
As PKZ Rev	TGGTTTGTCTGGTGTTGCAT

¹Primer amplifies SasaIFN-α1 and α2.