Table 1.

Primer names, sequences and annealing temperatures used for the characterization of barramundi MSTN-1 gene

Primer name	Sequence (5'-3')	Annealing temperature (°C)
Myo-up1	ATG CAT CYG TCT CAG ATT GTG [21]	52
Myo-up2	ATG AGC AYG CCA TCA CGG AGA	52
Myo-up3	CAA CTG GGG CAT CSA GAT TAA C	52
Myo-up5	GCT ACA AGG CCA ACT ATT GC	52
Myo-up8	ACA TAC AAC CTA TTA CAC	45
Myo-pro	CCA CAC GAT GGC RCT RTC	55
Jcu-R	GTT CAG TGG CCA TCA TCA T	52
Myo-L1	TTT CCC CTC GAA TCG AAG GC	52
Myo-L3	GCA ATA GTT GGC CTT GTA GCG	52
Myo-L5	TGA GGA TTC CTG GTT TCA CTC	52
Myo-L8	TGC TGG TGC GTT TCT TGG TC	55
Myo-Utr	GGC ATA TGT ATA CAA TAC	45
18S-F	TGGTTAATTCCGATAACGAACGA	54-45 step-down
18S-R	CGCCACCTGTCCCTCTAAGAA	54-45 step-down