Figure 7.

Mutational analysis of potential E2F1 binding sites. A) Point mutations introduced into the -742 promoter construct in order to eliminate possible E2F1 binding sites. B) The inducible E2F1-expressing Saos2 cells were transfected with the -742 promoter construct or the mutated versions thereof as indicated. Five hours after transfection, the medium was changed and cells were treated with doxycycline or were not treated. Data were normalized to β-galactosidase activity and are presented as the ratio relative to the activity of the unstimulated wild type construct. Luciferase activity of induced cells was significantly different from that in untreated cells (two-sided t test, p < 0.05, except for the double mutant) but there was no significant difference between wild type and mutants. Bars reflect the means +/- SD of 3 independent experiments.