Figure 3.

Enhanced EAU in IL-12p35 KO and in IFN-γ KO mice is associated with increased systemic and local IL-17 responses. WT and GKO mice on B10.RIII background (A–C) and WT and P35 KO mice on C57BL/6 background (D–F) were immunized with IRBP. On day 21 after immunization, eyes were collected for intracellular cytokine staining of eye-infiltrating inflammatory cells (A and D) or for histopathology (data pooled from two experiments with five mice per group; B and E). *, P < 0.05 versus WT. For FACS analysis, cells isolated from eyes were incubated either with PMA plus ionomycin and brefeldin A (PMA/Io) or with brefeldin A only (unstimulated). Shown are cells gated on CD4. Cytokine secretion from LN cells was measured by ELISA after 48 h of culture with IRBP.