Figure 5.
Th17 effector cells induce EAU in the absence of an IFN-γ response. Short-term Th17 lines were generated from IFN-γ KO mice immunized with p161–180 under Th17 conditions, as described in Materials and methods. (A) Intracellular IL-17 expression after a second stimulation with p161–180. IFN-γ expression is confirmed to be negative. (B) Supernatant from the stimulated cultures was tested for cytokine secretion by ELISA. (C and D) Pathogenicity of Th17 cells. Cells were collected from culture after the second cycle of stimulation, and 5 × 106/mouse were infused into WT or GKO recipients (five mice per group). On day 10 after transfer, eyes were collected for histopathology and disease was scored. Inset shows the inflammatory infiltrate, similar in WT and IFN–γ KO. Bar, 0.25 mm (0.02 mm in inset). A representative experiment of two is shown.