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. Author manuscript; available in PMC: 2008 Apr 10.
Published in final edited form as: Cell Cycle. 2007 Oct 2;7(1):96–105. doi: 10.4161/cc.7.1.5113

Figure 6.

Figure 6

Stabilization of Chf1 by mutation of ubiquitination sites. (A) A schematic diagram of Chf1 Lys to Arg mutants. Sites auto-ubiquitinated with Ubc4 are in gray. Sites additionally auto-ubiquitinated with Ubc13/Mms2 are in yellow. Sites that are trans-ubiquitinated by Chf2 are boxed. As shown by immunoblot (B) and relative quantitation versus GAPDH (C), individual Lys mutants such as Lys260Arg and Lys306Arg stabilize Chf2 protein alone and in combination with other Lys to Arg mutations, such as Lys237Arg, Lys240Arg, validating these residues as sites of biological regulation.