. Author manuscript; available in PMC: 2008 Apr 10.

Published in final edited form as: Cell Cycle. 2007 Oct 2;7(1):96–105. doi: 10.4161/cc.7.1.5113

TABLE 2.

Chf1 and Chf2 ubiquitination sites and linkages

Chf1 ubiquitinated peptides	sites	Ubc4+Chf1	Ubc13/Mms2+Chf1	Ubc4+Chf2
M^* DK^# HGLFSIR	K150	X
EAISK^# IPDQYHPVVFK	K204	X		X
THGCFK^# VDDQGNWFLK	K217	X
VDDQGNWFLK^# DVK	K237	X	X
DVK^# SSSGTFLNHQR	K240	X
LSSASTTSK^# DYLLHDGDIIQLGMDFR	K260	X
LK^# ANAFNK^# EALSR	K300, K306	X
ANAFNK^# EALSR	K306	X	X	X
IK^# NLQK^# LTTGLEQEDCSICLNK	K313, K317	X X
NLQK^# LTTGLEQEDCSICLNK	K317	X

LIFAGK^# QLEDGR	K48	X		X
TLSDYNIQK^# ESTLHLVLR	K63	X	X

Chf2 ubiquitinated peptides	Sites	Ubc4+Chf2	Ubc13/Mms2+Chf2	Ubc4+Chf1

NIVGGADGSTIVNNSQEMYK^# NLR	K211	X		X
K^# DKHGLFSIR	K256			X
KDK^# HGLFSIR	K258	X	X
K^# AGPGSQLVIGR	K288	X		X
DAISK^# IPEQYHPVVFK¹	K310	X	X	X
THGCFK^# VDSQGNWYIK	K333	X
VDSQGNWYIK^# DVK	K343	X		X
DVK^# SSSGTFLNHQR	K346	X	X
LSPASSLSK^# DTPLR¹	K366	X	X	X
LK^# ANSFNK^# EALQR	K406, K412	X X
ANSFNK^# EALQR	K412	X		X
LQNLQK^# LTTGIEEEDCSICLCK	K423	X
Ubiquitin peptides

LIFAGK^# QLEDGR	K48	X		X
TLSDYNIQK^# ESTLHLVLR	K63	X	X

Oxidation (+/− 15.99644 Da)

GlyGly (114.05421 Da)

Same K# detected on peptide with M* and/or longer peptide.