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. 2008 Apr 23;3(4):e2025. doi: 10.1371/journal.pone.0002025

Figure 4. The efficiency in generating DoxHPC lines directly correlates to the synergistic effect of Lhx2 expression on colony formation, and the generation and maintenance of the DoxHPC lines is optimal in SCF/IL-6. A.

Figure 4

Summary of the synergistic effect of Lhx2 expression (+Dox) on definitive hematopoietic colony formation of EB cells generated from the iLhx2 ES cells (high level of Lhx2 expression in 2 µg/ml of dox) or iLhx2-GFP ES cells (intermediate level of Lhx2 expression in 2 µg/ml of dox) replated in SCF/IL-6, or EB cells generated from the iLhx2 ES cells replated in SCF alone. * p<0,0001. B. Relative growth of a DoxHPC lines cultured in either IL-6, SCF/IL-6, or SCF. C. Relative expression of IL-6 analysed by real-time PCR comparing a DoxHPC line cultured either in SCF/IL-6 or in SCF for 8 days.