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. 2008 Apr 23;3(4):e2025. doi: 10.1371/journal.pone.0002025

Figure 5. Analyses of the effect of Lhx2 expression at different stages of ES cell differentiation.

Figure 5

A. Frequency of definitive CFCs in EB cells generated from the iLhx2 ES cells at the indicated days of differentiation starting at day 2,5 analysed in clonal assays in the absence (−Dox) or presence (+Dox) of dox. B. Frequency of CFC-Blast in day 3,25 EBs analysed in clonal assays with SCF and VEGF in the absence (−Dox) or presence (+Dox) of dox. C. Frequency of EryP precursor cells in day 6 EBs if dox is added during the indicated time points of ES cell differentiation and omitted in the clonal assays, compared to when no dox is added (Control) or if dox is added to clonal assays of control EBs (Control +Dox). The latter two control experiments are equivalent to the experiments shown in Figure 2A using the iLhx2 ES cells. D. Relative number EB cells recovered at day 6 of differentiation if dox is added to the iLhx2 ES cell line at the indicated time points during differentiation compared to when EBs are generated in the absence of dox (Control) which is arbitrarily set as 1. * p<0,005 compared to control. ** p<0,01 compared to control. E. Frequency of formation of secondary EBs in clonal assays of day 6 EBs if dox is added at the indicated time points during differentiation. Control EBs are generated without dox. F. Frequency of definitive CFCs in day 6 EBs when dox is added at indicated days of differentiation. Control EBs are generated without dox. G. Relative expression of the Brachyury gene during differentiation of the iLhx2 ES cell line revealing the progression of the gastrulation process in this particular ES cell line during differentiated in vitro.