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. 2008 Apr 23;3(4):e1982. doi: 10.1371/journal.pone.0001982

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Mok et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) The pie charts show the relative transcription level of each var-gene in the different clones. The original 3D7S8.4 clone (15 generations) dominantly expressed the PFD0630c, but sub-populations of other var-genes were also detected. In contrast, the var2csa gene was found to be highly expressed in 16 out of 17 sub-clones (3D7S8.4.2-3D7S8.4.17) but in one (3D7S8.4.1) generated from the long-term cultured ∼200 generations 3D7S8.4. (B) Expression levels of different var-genes in the 3D7S8.4 sub-clones, 3D7S8.4.1-17. var2csa was highly expressed in all the subclones except for 3D7S8.4.1 (•), which dominantly transcribed PF08_0103. var_common was expressed in all the parasites though at a relatively low level. (C) Northern-blot analysis of var2csa expression in total RNA extracted from the sub-clones 3D7S8.4.1, 3D7S8.4.2 and 3D7S8.4.17. The membrane was hybridized with a var2csa DBL1-2x probe. Comparable amounts of total RNA from each sample were loaded as shown in the ethidium bromide-stained gel (lower panel).