Table 1. Adhesion profile of 3D7AH1S2 and 3D7S8.4 at early and late generations.
Clones Generations | 3D7S8.4 | 3D7AH1S2 | ||
16 | >190 | 16 | >190 | |
Rosetting | ≈90% | - | ≈10% | - |
Autoagglutination | - | - | - | - |
CHO-CD36a | ≤250 | ≤10 | ≈500 | ≤10 |
CHO-ICAMa | ≤50 | ≤10 | ≤50 | ≤10 |
CHOa | ≤50 | ≤10 | ≤50 | ≤10 |
Soluble Heparinb | - | - | - | - |
Soluble CD31b | - | - | - | - |
CSAcd | - | - | - | - |
TSPc | - | - | ≈175 | - |
Placentae | ≈500 | - | - | - |
a: Number of IEs bound per 100 cells; b: % of IEs showing surface fluorescence when incubated with Alexa-labelled antigens; c: CSA or thrombospondin (TSP) -coated plastic (50 µg/ml); d: A postive control parasite FCR3CSA bound at ≈235±45 IEs/mm2; e: Number of IEs bound to 1 mm2 of placental tissue; -: Lack of detectable adhesion