TABLE 2.
τopen(ms)
|
|||||
---|---|---|---|---|---|
Mutant | τfast* | wfast† | τslow | wslow | τO-Avg (ms) |
mSlo(I) | 0.41 ± 0.36 | 0.22 ± 0.21 | 1.5 ± 0.55 | 0.78 ± 0.22 | 1.2 ± 0.10 |
I323A | 0.06 ± 0.05 | 0.07 ± 0.10 | 0.22 ± 0.07 | 0.93 ± 0.10 | 0.20 ± 0.05 |
I323C | 0.17 ± 0.25 | 0.33 ± 0.58 | 0.64 ± 0.15 | 0.67 ± 0.58 | 0.52 ± 0.05 |
I323E | 0.05 ± 0.01 | −0.02 ± 0.0 | 0.29 ± 0.01 | 1.0 ± 0.02 | 0.30 ± 0.01 |
I323F | 0.05 ± 0.01 | 0.01 ± 0.01 | 0.81 ± 0.11 | 0.99 ± 0.01 | 0.81 ± 0.08 |
I323K | 0.08 ± 0.01 | 0.19 ± 0.04 | 0.38 ± 0.06 | 0.81 ± 0.10 | 0.32 ± 0.01 |
I323L | 0.07 ± 0.04 | 0.16 ± 0.01 | 1.8 ± 0.03 | 0.84 ± 0.12 | 1.1 ± 0.09 |
I323Q | 0.13 ± 0.01 | 0.10 ± 0.02 | 0.48 ± 0.06 | 0.90 ± 0.07 | 0.46 ± 0.06 |
I323R | 0.20 ± 0.03 | 0.03 ± 0.01 | 0.32 ± 0.06 | 0.97 ± 0.09 | 0.31 ± 0.05 |
I323S | 0.21 ± 0.01 | 0.03 ± 0.01 | 0.44 ± 0.03 | 0.97 ± 0.01 | 0.43 ± 0.03 |
I323T | 0.18 ± 0.02 | 0.09 ± 0.06 | 0.51 ± 0.08 | 0.91 ± 0.06 | 0.27 ± 0.10 |
I323V | 0.05 ± 0.06 | 0.06 ± 0.05 | 0.22 ± 0.03 | 0.94 ± 0.05 | 0.21 ± 0.03 |
τ, time constant.
w, weight.