TABLE 2.
Maximum radius(μm) | Area (μm2) | Area fluctuation* (μm2) | Speed (μm/s) | Roundness | Persistence† | |
---|---|---|---|---|---|---|
Control | 25 (2/2) | 1080 (137/22) | 22 (11) | 0.31 (0.21/0.04) | 0.55 (0.08/0.06) | 0.77 (0.09/0.06) |
Nocodazole | 29 (4/3) | 1230 (277/42) | 42 (15) | 0.32 (0.12/0.04) | 0.45 (0.09/0.06) | 0.51 (0.13/0.13) |
Definitions of the six metrics are given in the section Calculation of metrics and calibration of the model. The measurements are performed on eight cells for each condition, and the recording period for each cell is 20 min at intervals of 0.8–1.5 s. These measurements yield average values over the recording period for each cell and the corresponding standard deviations where V represents the metrics. The averages and standard deviations of are the value outside the parentheses and the first term in the parentheses, respectively. The averages of are shown as the second term in the parentheses.
Area fluctuation for each cell is defined as the standard deviation of the area over the period of the observation, i.e., for the area. Therefore, it has the same unit as Area, and the same value as the second term in parentheses for Area. In addition, there is only one standard deviation for Area fluctuation, which is the standard deviation of for area.
Reference persistence lengths used, L, were 48.58 μm for the control and 38.87 μm for nocodazole-treated cells (see Calculation of metrics and calibration of the model, and Fig. S1).