TABLE 3.
Solubilities and free energies of hemoglobins studied with dextran
| Dextran | Predicted non-dextran | ||||
|---|---|---|---|---|---|
| HbS mutant | Co (g/dl) | Csat (g/dl) | Csat* (g/dl) | ΔΔG† (kcal/mol) | Reference |
| E6V(β)/L88A(β) | 7.68 | 6.86 | 26.4 | 0.24 | (10) |
| E6V(β)/K95I(β) | 9.90 | 8.97 | 30.4 | 0.32 | (10) |
| E6V(β)/L88A(β)/K95I(β) | 13.21 | 9.21 | 32.0 | 0.36 | (10) |
| E6V(β)/E121R(β) | 4.43‡ | 2.43 ± .03 | 15.4 ± .1 | −0.08 | (11) |
| D75Y(α)/E6V(β)/E121R(β) | 2.18‡ | 1.18 ± .04 | 9.5 ± .2 | −0.37 | (11) |
| D6A(α)/D75Y(α)/E6V(α)/E121R(β) | 1.70‡ | 0.70 ± .06 | 6.6 ± .4 | −0.59 | (11) |
| A19G(α)/A21G(α)/E6V(β) | 8.01 | 5.36 ± .07 | 23.9 ± .2 | 0.18 | (8) |
| A19Aib(α)/A21Aib(α)/E6V(β) | 7.70 | 2.78 ± .06 | 17.7 ± .2 | 0.00 | (8) |
| A19G(α)/A21G(α)/A78H(α)/E6V(β) | 7.26 | 2.80 ± .05 | 17.7 ± .2 | 0.00 | (8) |
| A19G(α)/A21G(α)/A78Q(α)/E6V(β) | 7.25 | 4.96 ± .14 | 22.8 ± .3 | 0.15 | (8) |
*
At 25°C.
†
ΔΔG relative to HbS.
‡
The initial concentration value is assumed as this value.