Table 2.
Primers used to amplify genomic sequence data.
| gene | amplicon | primer name | sequence | reference |
|---|---|---|---|---|
| COI | 820 bp | C1-J-2195 | TTGATTTTTTGGTCATCCAGAAGT | Simon et al., 1994 |
| TL2-N-3014 | TCCAATGCACTAATCTGCCATATTA | |||
| 16S rRNA | 512 bp | 16Sbr | CCGGTCTGAACTCAGATCACGT | Simon et al., 1994 |
| 16Sar | CGCCTGTTTAACAAAAACAT | |||
| wingless | 468 bp | wg1MP-F3 | GArTGyAArTGyCAyGGCATGTCsGG | M. Polihronakis, unpubl. |
| wg1MP-R3 | ACyICGCArCACCArTGGAAIGTGCA | |||
| 472 bp | Tsp'wg-f1 | ACnAThAArACnTGyTGGATGCGnCT | this study | |
| Tsp'wg-r1 | CrCArCACCArTGrAAnGTrCArAT | |||
| 28S rRNA | 1044 bp | D2-3665F (Bel28S) | AGAGAGAGTTCAAGAGTACGTG | Hancock et al., 1988 |
| D5-4749R (28SD4rev) | GTTACACACTCCTTAGCGGA | |||
| histone H3 | 330 bp | HexAF | ATGGCTACCAAGCAGACGGC | Ogden & Whiting, 2003 |
| HexAR | ATATCCTTGGGCATGATGGTGAC |