Table 2.

Summary of signal-to-noise ratios before and at the mutation position^a

Ex ptb	DNA sample			Signal-to-noise ratio
	Mutation Typec	Locationc	Size	No. of S/Nd	Mediane	Average	Minimumf	Maximumg
A	WT		80-nt single strand	60	17.4	150.0	1.4	>1000
B	A to T	6	80-nt single strand	18	15.0	67.7	4.1	>1000
C	Del G	7	80-nt single strand	21	10.5	106.2	1.7	>1000
D	A to C	3	80-nt single strand	9	9.8	121.7	5.2	>1000
F	WT		152-bp double strand	60	16.2	162.1	1.3	>1000
G	G to C	4	152-bp double strand	12	17.6	19.4	5.3	52.7
H	Heterozygous G to C	4	152-bp double strand	12	18.9	22.2	1.5	87.0

^adata are shown in Figure 3. In each set of four P*s, the signal-to-noise ratio is defined as the signal ratio between the matched P* and each of the three 3′ mismatched P*s.

^bpanel E is not included because the insertion is located ahead of the re-sequencing region.

^cthe mutation type is indicated on the sense strand. The mutation position is numbered assigning the first nucleotide in the target region as 1.

^dwith the wild type template, 60 signal-to-noise ratios were analyzed in 20 sets of P*s or 80 P*s. In Panel B with a mutant template, an A to T mutation is located at 6th nt of the re-sequencing region. Eighteen signal-to-noise ratios were analyzed in 6 sets of P*s.

^ethe difference between the average and median is large because many signal-to-noise ratios are >1000.

^fthe minimum signal-to-noise ratio is small because of high noise from contaminated unblocked primer. For example, the following spots had high noise at the mutation position: the ddT-Mut-P* at spot of row 3 column 5 on Panel A, the ddA-Mut-P* at spot of row 3 and column 3 on Panel B, the ddG-Mut-P* at spot of row 3 and column 6 on Panel C, the ddT-Mut-P* at spot of row 1 and column 9 on Panel D, the ddT-Mut-P* at spot of row 1 and column 9 on Panel G, and the ddT-Mut-P* at spot of row 1 and column 5 on Panel H.

^gA maximum of 1000 is taken.