Fig. 2.

Multiple alignments of Teashirt family members. Amino acids identical or similar in at least two of the proteins are shaded black or gray, respectively. Tc Tribolium castaneum; Ag Anopheles gambiae, Dm Drosophila melanogaster; Mm Mus musculus; Dr Danio rerio. a Multiple alignment of the three clustered zinc fingers. Zinc fingers (ZF) 1–3 are indicated above the sequence. Regions underscored with asterisks were concatenated and subjected to phylogenetic analysis. b Multiple alignment of the C-terminal zinc finger region. Zinc finger 4 is delimited above the alignment. Tsh lacks this zinc finger. c Distinct N-terminal motifs in dipteran Tsh family members (upper) and in Tribolium and vertebrates (lower)