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Tryparedoxin-dependent peroxidase activity in L. tarentolae clarified lysates. Metabolism of hydrogen peroxide (white bars), tBuOOH (gray bars), and CuOOH (black bars) in wild-type (WT), revertant (REV), and resistant (RES) extracts were measured in the presence of 1 μM recombinant L. major tryparedoxin. Data represent the means ± standard deviations from triplicate determinations. ***, P < 0.0001.
