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Immunoblot analysis of L. tarentolae whole-cell lysates. Blots of cell extracts from L. tarentolae wild-type (WT), revertant (REV), and Sb(III)-resistant (RES) cell lines (1 × 107 parasites in each lane) were probed with antisera to L. major TST, T. brucei T[SH]2 reductase (TryR), L. major T[SH]2 synthetase (TryS), L. major tryparedoxin (TryX), L. major tryparedoxin peroxidase type I (TryP), and L. major tryparedoxin peroxidase type II (TDPX).
