FIG. 3.
Cellular location of elevated tryparedoxin peroxidase. Subcellular fractions of L. tarentolae promastigotes containing the large-granule (LG), microsomal (MF), and cytosolic (C) fractions were prepared as described in Materials and Methods. Western blots of these equally loaded fractions (30 μg of protein in each lane) were probed with antiserum to L. major tryparedoxin peroxidase (type I). In addition, blots were stripped and reprobed with antiserum to marker proteins for each subcellular fraction to demonstrate the purity of each fraction (LG, anti-Hsp60; C, anti-L. major T[SH]2 synthetase; MF, anti BiP).