TABLE 1.
Intracellular thiol levels in Sb(III)-sensitive L. tarentolae TarII (wild type), Sb(III)-resistant TarII Sb1.1, and partially resistant revertant TarII Sb1.1rev
| Cell line | EC50 (μg ml−1) | Thiol content (nmol 108 cells−1)a
|
|||
|---|---|---|---|---|---|
| GSH | GspdSH | T[SH]2 | Total SH | ||
| TarII (wild type) | 7.35 ± 0.14 | 0.75 ± 0.04 | 0.325 ± 0.08 | 1.6 ± 0.03 | 4.28 |
| TarII Sb1.1rev (revertant) | 56.5 ± 1.9c | 0.8 ± 0.05c | 0.37 ± 0.02c | 1.70 ± 0.07c | 4.56c |
| TarII Sb1.1 (resistant) | 377 ± 38.4d | 8.76 ± 0.11d | 1.4 ± 0.01d | 10.05 ± 0.1d | 30.26d |
| TarII Sb1.1 − Sbb | Not applicable | 11.1 ± 0.2d | 1.8 ± 0.09d | 12.1 ± 0.15d | 37.1d |
a
Thiol levels represent the means ± standard deviations from triplicate determinations.
b
Cells grown in the absence of Sb(III) for two passages prior to analysis.
c
Not significantly different from wild-type values (P > 0.05).
d
Significantly different from wild-type value (P < 0.0001).