TABLE 2.
Specific activities of T[SH]2 reductase, TST, and T[SH]2-dependent peroxidase in L. tarentolae soluble extracts
| Enzymatic activity | Substrate | Sp act (nmol min−1 mg−1)a
|
||
|---|---|---|---|---|
| Wild type | Revertant strain | Resistant strain | ||
| T[SH]2 reductase | T[S]2 | 112 ± 9 | 120 ± 9b | 114 ± 7.9b |
| TST | CDNB | 1.2 ± 0.5 | 1.6 ± 0.8b | 1.0 ± 0.4b |
| T[SH]2-dependent peroxidase | H2O2 | 3.5 ± 0.4 | 14.4 ± 0.9c | 25.7 ± 1.0c |
| CuOOH | 1.0 ± 0.1 | 3.1 ± 0.1c | 8.9 ± 0.4c | |
| tBuOOH | 1.9 ± 0.2 | 8.8 ± 0.2c | 22.4 ± 0.4c | |
a
All enzymatic activities were assayed as described in Materials and Methods and were corrected for nonenzymatic background rates. Values are means ± standard deviations.
b
P > 0.05 compared to wild-type value.
c
P < 0.0001 compared to wild-type value.