TABLE 3.
Comparison of Sb(III) sensitivity and tryparedoxin peroxidase levels in wild-type, revertant, and transgenic L. tarentolae cell lines
| Cell Line | Vector | Relative protein levela | Mean ± SD
|
|
|---|---|---|---|---|
| Peroxidase activity (nmol min−1 mg−1) | EC50 (μg ml−1) | |||
| Wild type | None | 1 | 3.5 ± 0.4 | 7.3 ± 0.4 |
| pIR1SAT | 1 | 4.3 ± 0.2 | 9.3 ± 0.4 | |
| pIR1SAT-LmTryP | 2.2 | 8.3 ± 0.6b | 22.7 ± 0.8b | |
| pIR1SAT-LmTryP (R128D) | 3.3 | 4.4 ± 0.1 | 8.3 ± 0.5 | |
| Revertant | None | 2.8 | 14.4 ± 0.9b | 56.5 ± 1.9b |
a
As determined by densitometry of Western blots and compared to wild-type level.
b
P < 0.0001 compared to wild-type value.