TABLE 1.
Predicted drug activity on liver stage of P. yoelii yoelii
| Drug (therapeutic category) | DF1a (class) | DF2b (class) | IC50 expc (nM) | TC50 expd (nM) | SI | Log Pe |
|---|---|---|---|---|---|---|
| Active drugs | ||||||
| Monensin (antibacterial/ionophore) | 4.22 (A) | −21.62 (NC) | <10−3 | 3.20 × 104 | >3.2 × 107 | 2.77 |
| Nigericin (ionophore) | 3.36 (A) | −24.22 (NC) | <10−3 | 1.32 × 104 | >1.32 × 107 | 3.78 |
| Delaverdine (antiviral) | −0.21 (NC) | 6.75 (HA) | 0.846 | >1 × 105 | >1.18 × 105 | 3.65 |
| Mibefradil (antihypertensive) | 7.59 (A) | 6.66 (HA) | 0.873 | 6.79 × 104 | 7.7 × 104 | 3.47 |
| Licochalcone A (estrogenic flavonoid) | 8.26 (A) | 7.99 (HA) | 0.927 | 6.34 × 104 | 6.83 × 104 | 2.57 |
| Miconazole (antifungal) | 1.46 (A) | 1.77 (HA) | 2.03 | 7.04 × 104 | 3.47 × 104 | 5.94 |
| Dobutamine (cardiotonic) | 5.74 (A) | 1.52 (HA) | 3.7 | >1 × 105 | >2.70 × 104 | 1.57 |
| Ritonavir (antiviral) | 8.80 (A) | −5.38 (A) | 34.2 | 6.73 × 104 | 1.96 × 103 | 6.71 |
| Saquinavir (antiviral) | 9.14 (A) | −6.76 (A) | 35.2 | 6.37 × 104 | 1.80 × 103 | 4.96 |
| Epoximicin (antineoplastic) | 8.17 (A) | 7.92 (HA) | 3.95 × 103 | 4.73 × 104 | 11.97 | 2.38 |
| Indinavir (antiviral) | 8.89 (A) | −10.55 (A) | 5 × 103 | 8.77 × 103 | 1.754 | 5.94 |
| Vinblastine (antineoplastic) | 1.21 (A) | 38.71 (NC) | 7.95 × 103 | 5.45 × 104 | 6.85 | 3.55 |
| Nordihydroguaiaretic acid (antineoplastic) | 9.03 (A) | 2.88 (HA) | 3 × 104 | >1 × 105 | >3.33 | 1.93 |
| Inactive drugs | ||||||
| Fenbendazole (antihelminthic) | −3.33 (I) | 3 × 104 | >1 × 105 | >3.33 | 3.63 | |
| Quinacrine (anthelminthic/antimalarial) | −0.93 (I) | 3 × 104 | >1.16 × 104 | >0.38 | 3.74 | |
| Rimandine (antiviral) | −2.69 (I) | 35.6 | >1 × 105 | >3.33 | 3.85 | |
| Thiophanote (anthelminthic) | −4.04 (I) | 3 × 104 | >1 × 105 | >3.33 | 1.93 | |
| Reference drugs | ||||||
| Atovaquone | 9.37 (A) | 3.63 (HA) | 57 | 16.5 × 103 | 289 | 3.67 |
| Primaquine | 0.91 (A) | 1.79 (HA) | 75.7 | 12.5 × 103 | 165 | 1.73 |
a
From discriminant function DF1.
b
From discriminant function DF2. IC50 exp, experimental IC50; TC50 exp, experimental TC50; log P calc, calculated log P.
c
Evaluation of antimalarial activity in vitro against the liver stage of P. yoelii yoelii as described previously by Mahmoudi et al. (17, 18).
d
Assessment in vitro by MTT assay.
e
Value of log P calculated with the MLR equation.