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. 2003 Nov;77(21):11425–11435. doi: 10.1128/JVI.77.21.11425-11435.2003

FIG. 1.

FIG. 1.

Chemical induction of ORF50 promoter. (A) BCBL-1 cells were treated with TSA, NaB, or TPA and analyzed by RT-PCR for expression levels of ORF50 (top panel) or GAPDH (lower panel). Controls lacking reverse transcriptase (−RT) are indicated for ORF50. (B) The ORF50 promoter fused to luciferase (p50) or the pGL3-Basic control plasmid were transfected into BCBL-1 cells and treated with TSA, NaB, or TPA. (C) BCBL-1 cells were transfected with luciferase reporter plasmid pGL3-Basic or promoters derived from BZLF1, ORF50 (p50), the K8 immediate early promoter (K8-IE), or the K8 delayed early promoter (K8-DE). (D) p50 and pGL3-Basic control plasmids were transfected into JSC-1, BCBL-1, 293, or DG75 cells. Luciferase activity was measured as induction (n-fold) upon treatment with 3 mM NaB for 24 h.