TABLE 1.
Effect of disrupting ampR on β-lactamase induction in S. maltophilia K279a
| Strain | Inducer added? | L1-specific activitya | L2-specific activity |
|---|---|---|---|
| Wild-type K279a | No | 0.04 | 0.07 |
| Wild-type K279a | Yesb | 0.49 | 0.46 |
| K279a ampRFS | No | 0.08 | 0.08 |
| K279a ampRFS | Yes | 0.01 | 0.01 |
a
Specific activity values were calculated as described previously using nitrocefin as the substrate (7). Data represent averages of the results of three separate experiments with <20% variation.
b
Nutrient broth-grown cultures were induced at an OD600 of 0.4 for 2 h with 10 mg/liter imipenem.