FIG. 4.

β-Galactosidase reporter activity of lacZ reporter fusion integrants of AS and AR isolates during growth phase. Transcriptional fusion (P_CDR1-lacZ) and translational fusion (P_CDR1-CDR1-lacZ) reporter transformants of each isolates were grown from an initial OD₆₀₀ of 0.1 in YEPD broth and withdrawn at the indicated time points of growth for β-galactosidase reporter activity (Fig. 4A, B, C, and D). The inset depicts growth curves of the P_CDR1-lacZ (□) and P_CDR1-CDR1-lacZ (▪) reporter transformants in AS and AR isolates. The negative-control parental strain (without lacZ fusion constructs) reporter activity value was always below 0.5 Miller units, and it was subtracted from the reporter activity of each corresponding transcriptional and translational fusion transformant. The values are means ± standard deviations (indicated by the bars) for three independent experiments with duplicate measurements of two independent clones. Gu4 transformants (A), Gu5 transformants (B), DSY294 transformants (C), and DSY296 transformants (D) were analyzed. Empty and filled bars indicate transcriptional (P_CDR1-lacZ) and translational fusion (P_CDR1-CDR1-lacZ) transformants in both AS and AR backgrounds.