FIG. 2.

Effect of the 14-membered-ring macrolide RXM (A), EM (B), or CAM (C) on the SLIGKV-NH₂-induced production of IL-8 in NHEK. Empty bars, control; filled bars, SLIGKV-NH₂-treated cells. An analysis of variance followed by Scheffe's test was performed for the statistical analysis of data. *, P < 0.001; **, P < 0.01; ***, P < 0.05; ns, not significant. (D) Effect of RXM, EM, or CAM on the viability of NHEK. NHEK were incubated with no macrolide (empty bars) or 10 μM (gray bars) or 100 μM (filled bars) RXM, EM, or CAM for 72 h, and then cell viability was examined. None of those 14-membered-ring macrolides reduced the viability of NHEK.