TABLE 1.

GT-IR genes and their corresponding functions

Gene	Median MICa (μg/ml)					Radius (mm) of zone of growth inhibition (mean ± SD)b	Presence of human homolog	Growth rate (%) (mean ± SD)	Function(s) [reference(s)]
	CAS	AMB	FLC	Cycloheximide	GT
SNF1	0.007	0.5	16.0	0.025	8	15.0 ± 0.70	+	86 ± 3	General metabolic pathways/biosynthesis, AMP-activated serine/threonine kinase (26)
IFA38	0.007	0.5	16.0	0.025	2	19.7 ± 0.3	+	93 ± 5	General metabolic pathways/biosynthesis, microsomal beta-keto-reductase (1)
OPI1	0.007	0.5	16.0	0.025	2	16.8 ± 2.5	−	100 ± 5	General metabolic pathways/biosynthesis, negative regulator of phospholipid biosynthesis (25)
RTG2	0.007	0.5	16.0	0.025	8	21.2 ± 0.3	+	96 ± 4	Mitochondrial function/transcriptional activator of RTG and TOR pathways (5, 15)
APL2	0.007	0.5	16.0	0.025	8	21.5 ± 0.3	+	99 ± 3	Vesicular transport, protein processing/beta-adaptin (27)
RAD18	0.007	0.5	16.0	0.025	8	21.5 ± 0.7	+	92 ± 4	DNA damage repair/postreplication repair (17, 21)
YGL235W	0.007	0.5	16.0	0.025	8	19.6 ± 3.6	−	101 ± 4	Unknown function/potential Cdc28 substrate
YOR345C	0.007	0.5	16.0	0.025	16	19.1 ± 1.5	−	97 ± 5	Unknown function
YLR456W	0.007	0.5	16.0	0.025	8	19.5 ± 0.7	+	100 ± 3	Unknown function
YGL072C	0.007	0.5	16.0	0.025	16	24.5 ± 0.7	−	93 ± 5	Unknown function

^aThe median MICs (μg/ml) of the BY4741 (wt) strain to CAS, AMB, FLC, cycloheximide, and GT were 0.007, 0.125, 32, 0.025, and 0.5, respectively, as determined by broth microdilution susceptibility testing in both inocula tested (∼10⁵ and ∼10³ yeast cells/well).

^bThe disk diffusion method showed that the radius of the zone of growth inhibition of the BY4741 strain to H₂O₂ (21.0 ± 0.7 mm) was comparable to those of the GT-IR and GT-IS strains (P was not significant for all comparisons).