TABLE 2.
GT-IS genes and their corresponding functions
| Gene | Median MICa (μg/ml)
|
Radius (mm) of zone of growth inhibition (mean ± SD)b | Presence of human homolog | Growth rate (%) (mean ± SD) | Gene function(s) (reference) | ||||
|---|---|---|---|---|---|---|---|---|---|
| CAS | AMB | FLC | Cycloheximide | GT | |||||
| CYS3 | 0.028 | 0.25 | 32.00 | 0.025 | 0.06 | 22.7 ± 0.3 | + | 94 ± 6 | General metabolic pathways, biosynthesis/cystathionine gamma-lyase (18) |
| MEF2 | 0.028 | 0.25 | 32.00 | 0.025 | 0.06 | 20.9 ± 0.1 | + | 105 ± 3 | Mitochondrial function/transcriptional elongation factor (20) |
| YKL037W | 0.028 | 0.25 | 32.00 | 0.025 | 0.125 | 21.8 ± 0.2 | − | 101 ± 4 | Unknown function |
a
The median MICs (μg/ml) of the BY4741 (wt) strain to CAS, AMB, FLC, cycloheximide, and GT were 0.007, 0.125, 32, 0.025, and 0.5, respectively, as determined by broth microdilution susceptibility testing in both inocula tested (∼105 and ∼103 yeast cells/well).
b
The disk diffusion method showed that the radius of the zone of growth inhibition of the BY4741 strain to H2O2 (21.0 ± 0.7 mm) was comparable to those of the GT-IR and GT-IS strains (P was not significant for all comparisons).