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. 2003 Nov;77(21):11459–11470. doi: 10.1128/JVI.77.21.11459-11470.2003

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Copyright © 2003, American Society for Microbiology

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FIG.2. — IN chimeric virus technology. (A) Construction of the pNL4.3 clone with the IN gene deleted. To generate the clone with the IN gene deleted, the proviral molecular clone pNL4.3 was digested with AgeI and Van91I. A linker sequence containing the XbaI restriction site was ligated into the vector. (B) Construction of HIV strains with recombined IN genes. For IN recombination experiments, MT-4 cells were cotransfected with the XbaI-linearized clone in which the IN gene had been deleted and MW1-MW2 PCR product. ds, double-stranded.

FIG.2. — IN chimeric virus technology. (A) Construction of the pNL4.3 clone with the IN gene deleted. To generate the clone with the IN gene deleted, the proviral molecular clone pNL4.3 was digested with AgeI and Van91I. A linker sequence containing the XbaI restriction site was ligated into the vector. (B) Construction of HIV strains with recombined IN genes. For IN recombination experiments, MT-4 cells were cotransfected with the XbaI-linearized clone in which the IN gene had been deleted and MW1-MW2 PCR product. ds, double-stranded.

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