TABLE 1.
Genotypic analysis of the IN genes from HIV-1(IIIB) strains selected in the presence of L-708,906 relative to the wild-type HIV-1(IIIB) strain
| HIV-1 straina | Amino acid at position
|
||
|---|---|---|---|
| 66 | 74 | 230 | |
| HIV(IIIB) | T | L | S |
| IIIB/L-708,906(#25) | T/Ic | L | S |
| IIIB/L-708,906(#35) | I | L/M | S |
| IIIB/L-708,906(#40) | I | M | S/R |
| IIIB/L-708,906(#60) | I | M | R |
| IIIB/L-708,906(#70) | I | M | R |
| IIIB/L-708,906(#60+#25 w/o L-708,906)b | I | M | R |
| Mutation type | Transition (ACA→ATA) | Transversion (CTG→ATG) | Transversion (AGC→AGA) |
a
HIV-1(IIIB) strains selected in MT-4 cells in the presence of L-708,906 during a certain number of passages (25, 35, 40, 60, or 70 passages).
b
HIV-1(IIIB) strain selected in MT-4 cells in the presence of L-708,906 during 60 passages, followed by 25 additional passages of this virus in the absence of L-708,906.
c
Part of the virus population (at least 30%) contains a certain mutation, while the wild-type amino acid is still present at this position in the rest of the population as determined by population sequencing.