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. 2008 Feb 1;74(7):1988–1996. doi: 10.1128/AEM.01851-07

FIG. 3.

FIG. 3.

Transcriptional analysis of aldB expression. (A) RT-PCR analysis of aldB mRNA levels in CRL264 grown in M17G or M17GC medium at pH 5.0 or 7.0. The results are depicted as in Fig. 2. (B) Organization of the Lactococcus bv. diacetylactis leu-ilv-ald cluster, with a schematic representation of pIL91 and the β-galactosidase activity encoded by IL1403(pIL91) grown in M17G at the indicated pHs. In the sequence of P3 promoter region, the upper lines indicate the −10 extended box and the transcriptional start site (+1) of aldB mRNA identified by Goupil-Feuillerat (8). The translational start site is underlined with a discontinuous line. The sequence TAA located 60 nt upstream of −10 extended box indicates the stop codon of ilvA gene. The location and size of the fragment fused to lacLM in pIL91 are also shown.